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Botox Dilution and Reconstitution Chart

A one-page reference for the treatment room: how many units are in each 0.1 mL for common vial sizes and saline volumes, with the Botox Cosmetic label's own dilutions marked. Under the chart is a record for each vial you reconstitute: date, lot, volume, concentration and use-by.

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Botox Dilution and Reconstitution Chart

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Arithmetic only. Units are specific to each brand. The product label governs diluent, volume, storage and use-by time.

Dilution chart

Vial size
Saline added
Units per mL
Units per 0.1 mL
Units per 0.05 mL
Note
50-unit vial
50 units
1 mL
50
5
2.5
50 units
1.25 mL
40
4
2
Botox Cosmetic label
50 units
2.5 mL
20
2
1
100-unit vial
100 units
1 mL
100
10
5
100 units
2 mL
50
5
2.5
100 units
2.5 mL
40
4
2
Botox Cosmetic label
100 units
4 mL
25
2.5
1.25
100 units
5 mL
20
2
1
200-unit vial
200 units
2 mL
100
10
5
200 units
4 mL
50
5
2.5
200 units
5 mL
40
4
2
200 units
8 mL
25
2.5
1.25

Units per 0.1 mL = units in the vial ÷ (mL of saline × 10). Example: 100 units in 2.5 mL = 100 ÷ 25 = 4 units per 0.1 mL.

Reconstitution record

Date and time
Product
Lot #
Vial units
Saline mL
Units / 0.1 mL
Reconstituted by
Use by (per label)

How to read the chart

Find the vial size, then the volume of saline you add. The row tells you how many units are in each mL, each 0.1 mL and each 0.05 mL of the reconstituted product. The formula is simple: units per 0.1 mL equals the units in the vial divided by ten times the mL of saline.

The Botox Cosmetic prescribing information reconstitutes a 100-unit vial with 2.5 mL, or a 50-unit vial with 1.25 mL, of preservative-free 0.9% sodium chloride, giving 4 units per 0.1 mL. Those rows are marked on the chart.

Things the chart can't tell you

  • Units aren't interchangeable between brands. A unit of one botulinum toxin product is not the same as a unit of another, so never convert doses between brands with this chart.
  • The diluent, volume, handling, storage and how long reconstituted product can be kept all come from the product's label. Write the use-by on the vial and in the record.
  • Dosing is a clinical decision for the injector and the medical director, not something a dilution chart decides.

Why keep a reconstitution record

Writing down the lot number, volume and time for each vial means every treatment can be traced to a vial if there's a recall, an adverse event or a question about a product's source. It also shows an inspector that reconstituted product isn't kept past its use-by.

Questions

How many units are in 0.1 mL of Botox?

It depends on how much saline was added. A 100-unit vial reconstituted with 2.5 mL, the dilution on the Botox Cosmetic label, gives 4 units per 0.1 mL. With 2 mL it's 5 units per 0.1 mL, and with 1 mL it's 10.

Does this chart work for Dysport, Xeomin or other brands?

The arithmetic works for any vial, but units aren't interchangeable between products and each label has its own reconstitution instructions. Use the chart only for the vial size and volume you actually used, and follow that product's label.

Sources

General guidance, not legal or medical advice. Requirements vary by state; follow your medical director and product labels.